Background:
Probiotic therapy in the form of food formulations is a natural and cost-effective strategy for gut health. At NDRI, our group was the first to focus on indigenous strains of probiotic lactobacilli of Indian gut origin. Amongst these, one of the strains belongs to Lactobacillus plantarum Lp91 whose identity has been established by molecular tools viz. 16S rRNA (GQ922598) sequencing as well as housekeeping genes pheS (KC509913.1), tkt4 (KC509921.1), pgm (KC509919.1), gyr (KC509917.1), mutS (KC509918.1), gdh (KC509916.1), ddl (KC509915.1) and purK1 (KC509920.1) genes. The draft Genome Sequence of L.plantarum Lp91 has been submitted to the NCBI gene bank under the accession number AXDQ0000000. The probiotic strain has been assessed for safety in both in-vitro and in vivo toxicity in animal models and deposited at Microbial Type Culture Collection and Gene Bank (MTCC), housed at the Institute of Microbial Technology (IMTECH), Chandigarh under Budapest treaty with MTCC No.5690. The probiotic attributes and functional efficacy of Lp91 has been established both in vitro (cell culture) and in vivo (animal model). It possesses high tolerance to acidic pH and bile, strong adhesion, Bsh activity, hypocholerolemic effect, anti-inflammatory and immuno-modulatory both in HT-29 cell line and mice besides being effective in amelioration of colitis in mouse model. As a scientifically established probiotic strain. L. plantarum Lp91 is an ideal candidate probiotic for developing probiotic fermented dairy products and in the form of food supplements as powders and sachets for effective management of health care and well-being in today’s scenario when consumer is looking for foods for nutrition and well-being to cut down on medicine
Technology Details:
As a scientifically established probiotic strain. L. plantarum Lp91 is an ideal candidate probiotic for developing probiotic fermented dairy products and in the form of food supplements as powders and sachets for effective management of health care and well-being (L. plantarum is listed in the schedule VII (List of strains as probiotics) in the Standards of Health supplements and Nutraceuticals) in today’s scenario when consumer is looking for foods for nutrition and well-being to cut down on medicine.